The speed of these replies is crazy, thanks a lot. I obviously didn't make it clear enough what I meant, sorry about that..i tried! I have made an image, because I'm not particularly good at describing things! Sorry about sticking 'help' in the title, I just assumed I should be specific in the general area.
I'm not always entirely clear why, or when, I end up with a lot of invisible rubbish...but I have a lot of sprites that I am trying to keep as small as possible and also there are elements that I wish to remove for various reasons. But in opening them into MSpaint I noticed that there is all this clutter, but there is no easy way that I can see of removing it. (in the example attached it would be easy as its 100% and 0% opaque, but usually this isn't so)
In the example I have the file opened to paint.net, then it opened to MSpaint, and then how I wish to open it in MSpaint and see it.
Pyrochild: Your answer in explaining what I meant is basically correct. I'm not specifically trying to remove the transparent pixels entirely, but rather just blank them. I have no idea how the file saving works, but if there is a colour that is default and therefore doesn't need stating then to make it that colour would be good.
cheers,