David Thiessen

Help isolating the red from blood vessel pictures

Recommended Posts

Hello everyone!

 

This might be a little unconventional. I'm a researcher and I need to isolate the red from some pictures I took (samples bellow). The problem is that the red of the vessels is not strong enough for the quantification software (AngioTool)  to identify what's a vessel and whats the background. It doesn't have to be red, It just need to be different enough from the background. I've been messing with the luminosity with the plugin Curves+  but is not enough.

 

Any help of how could I do this? Any help would be really appreciated! The second picture is similar to what I need. 

 

 

sample.png

sample3-min_optimized.png

Edited by David Thiessen

Share this post


Link to post
Share on other sites

I suggest using the Kill Color Keeper plugin.

Choose the color picker, choose the 5x5 pixels sampling at the top, click somewhere on the background and run the plugin. Then choose the white color and run the plugin again.

This should clear most of the background noise.

Now pick the eraser, reduce the hardness at the top to 0 and the opacity to 128, and choose a large brush size. Then you can erase the leftovers manually. Add a black layer at the bottom and you're done.

I did this with your first image and I'm attaching what I got.

Sadly I don't think you can automate this easily.

sample.jpg

Share this post


Link to post
Share on other sites

I'd be more inclined to go with Blend Modes rather than eraser etc.  I think you're going to lose some of the small surrounding capillaries regardless, as they're so close in colour to the background, (but you could use the line tool to replace them ?))

 

vessels-1.png

 

With the original image on top of the merged layers above

 

Vessels-2.png

Edited by welshblue

Share this post


Link to post
Share on other sites

Join the conversation

You can post now and register later. If you have an account, sign in now to post with your account.

Guest
Reply to this topic...

×   Pasted as rich text.   Paste as plain text instead

  Only 75 emoji are allowed.

×   Your link has been automatically embedded.   Display as a link instead

×   Your previous content has been restored.   Clear editor

×   You cannot paste images directly. Upload or insert images from URL.