Process:
print screen of the website I am developing
paste into paint.net
Cut a section out and save it as a PNG (tried 32-bit and 8-bit)
Add the new image to the website
The new image colour is "off" by a smallish amount (supposed to be the same colour as the background that I cut it from)
Open paint.net, notice the colour somehow changed. Use the fill tool to change it to what it was supposed to be
Still doesn't fix the problem even though the eye dropper is reporting the same colour as the colour used by the website (as seen in css hex)
Installed photoshop, performed the same process as above (without the extra step of filling in the mysteriously changed colour). Works as expected (image is same colour as website background).