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Hi MJW, Thank you for your advice. I'll check if it's the color issue in my code. Thank you very much.

Hello MJW, Although the two images seem to be the same ,data of these two are not the same when I print out the log at the same location of the buffer like : data[100], data[1000], data[1500]...etc. It does confuse me at this part... It's all before the function to show the segmeation images on the tool. But I will chek my code. Thank you very much.

Hello MJW, Sorry for the unclear description. I mean the preview icon of these two images look the same. But when use my own tool(MFC tool) to load the bitmap data into a buffer and then show on my picture control item. It looks more darker than loading the original one. Again, I apology for the unclear description... Thank you very much.

Hello toe_head2001, These images are all Bitmap filetype . Thank you very much.

Hello, I uesd the paint.net to load an 8-bit image (called it A). And save it as a new 8-bit image file (called it B). The look of these two are seem the same. But after I read the hex data of them, their data are not the same. For example, the offsetvalue (10th byte) of A is 1078, but 474 for B. And after reading all the bitmap data and show them on my own tool. The B's picture is more darker than the A one. Can anyone tell me why there's a difference after saving image without any editing? Thank you very much.